›› 2011, Vol. 23 ›› Issue (2): 107-110.doi: 10.3969/j.issn.1004-616x.2011.02.006

• 论著 • 上一篇    下一篇

p-Stat3、VEGF-C和MMP-2在非小细胞肺癌中的表达及其与淋巴结转移的关系

王凤云，张艳桥*，罗 明，武 云，李 鹏，李志伟，孙 伟   

  1. 包头医学院第三附属医院肿瘤科，内蒙古 包头 014030
  • 收稿日期:2010-10-09 修回日期:2010-11-17 出版日期:2011-03-30 发布日期:2011-03-30
  • 通讯作者: 张艳桥

The expressions of p-Stat3 , VEGF-C and MMP-2 in non-small cell lung cancer and their relationship with lymph node metastasis

WANG Feng-yun, ZHANG Yan-qiao*, LUO Ming, WU Yun, LI Peng, LI Zhi-wei, SUN Wei   

  1. The Affliliated Third Hospital of Baotou Medical College, Baotou 014030, Inner Mongolia, China
  • Received:2010-10-09 Revised:2010-11-17 Online:2011-03-30 Published:2011-03-30
  • Contact: ZHANG Yan-qiao

PDF

1550

摘要： 检测非小细胞肺癌组织中磷酸化信号转导和转录激活子-3 (p-Stat3)、血管内皮生长因子C(VEGF-C)和基质金属蛋白酶2( MMP-2)的表达情况，探讨p-Stat3与VEGF-C、MMP-2之间，以及这三者与临床病理指标之间的相关性。 方法: 采用免疫组织化学SP法，检测53例非小细胞肺癌组织和10例正常肺组织中p-Stat3、VEGF-C和MMP-2的表达情况, 并对p-Stat3、VEGF-C和MMP-2的表达情况进行相关性分析。 结果: 在53例非小细胞肺癌病例中，p-Stat3、VEGF-C和MMP-2的阳性表达率分别为45.2%(24/53)、77.3%(41/53)和58.4%(31/53)。其中，p-Stat3的表达在腺癌中最显著，阳性表达率为75%(9/12)，且在低分化肺癌组织中的表达明显高于高分化肺癌组织( P＜0.01)，在有淋巴结转移的肺癌组织中高于无淋巴结转移的肺癌组织( P＜0.05)，而阳性表达率与患者的年龄、性别和肿瘤的大小无明显相关 ( P＞0.05)。VEGF-C和MMP-2在有淋巴结转移的非小细胞肺癌组织中的阳性表达率高于无淋巴结转移的肺癌组织( P＜0.05)，且VEGF-C、MMP-2的表达与p-Stat3的表达呈正相关(依次为r = 0.427和0.345,P均＜0.05)。 结论: 在非小细胞肺癌组织中，p-Stat3、VEGF-C和MMP-2表达均上调，高表达的p-Stat3与肺癌的发生、发展及淋巴结转移密切相关，其机制可能与VEGF-C和MMP-2有关。

关键词: 非小细胞肺癌, p-Stat3, VEGF-C, MMP-2, 免疫组织化学, 淋巴结

Abstract: To examine the expressions of p-Stat3, VEGF-C and MMP-2 and to study their relationship. METHODS: P-Stat3, VEGF-C and MMP-2 proteins were detected in formalin-fixed paraffin-embedded tissue specimens from 53 non-small cell lung cancer tissues and 10 normal lung tissues by SP immunohistochemical method. The levels of their expression and their relationship with p-Stat3, VEGF-C and MMP-2 were analyzed. RESULTS: In 53 non-small cell lung cancers, p-Stat3, VEGF-C and MMP-2 was expressed in 45.2%(24/53), 77.3%(41/53) and 58.4%(31/53), respectively. p-Stat3 expression was more pronounced in adenocarcinoma at 75%(9/12). p-Stat3 expression was much higher in the poorly differentiated than the well differentiated tissues (P ＜0.01). p-Stat3, VEGF-C and MMP-2 expressions were much higher in the non-small cell lung cancers with lymph node metastasis than those without(P＜0.05). There was no significant correlation with the size of tumor, age and sex of the patients(P＞0.05). There was also positive correlation between the expressions of p-Stat3 and VEGF-C, MMP-2 in non-small cell lung cancer(P＜0.05). CONCLUSION: p-Stat3, VEGF-C and MMP-2 were upregulated in non-small cell lung cancer. Over-expression of p-Stat3 in the non-small cell lung cancer development and metastases may be related to VEGF-C and MMP-2.

Key words: non-small cell lung cancer, p-Stat3, VEGF-C, MMP-2, immunohistochemical method, lymph node

版权所有 ©2019 《癌变·畸变·突变》编辑部 E-mail： office@egh.net.cn
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持：support@magtech.com.cn
粤ICP备 10216025号